题　目：Optoelectronic Tweezers: Massively Parallel Manipulation of Single Cells and Nanoscale Particles using Direct Optical Images

报告人：Dr. Pei-Yu Chiou

Department of Mechanical and Aerospace Engineering

University of California at Los Angeles

主持人：席建忠

时　 间：2007年3月29日（周四）下午6：30

地 　点：北京大学理科2号楼 2129

 

Abstract:

The ability to manipulate biological cells and nanometer-scale particles plays an important role in many biological and colloidal science applications. However, conventional manipulation techniques, including optical tweezers, electrokinetic forces (electrophoresis, dielectrophoresis (DEP), and traveling-wave dielectrophoresis), magnetic tweezers, acoustic traps, and hydrodynamic flows, cannot achieve high resolution and high throughput at the same time. Optical tweezers offers high resolution for trapping single particles, but has a limited manipulation area due to tight focusing requirements, while electrokinetic forces and other mechanisms provide high throughput, but lack the flexibility or the spatial resolution necessary for controlling individual cells. We present an optical image-driven electrokinetic technique that permits sculpting high-resolution electric fields potential landscape on a photoconductive surface for manipulating single cells, nanoparticles, and macromolecules such as DNA. It requires 100,000 times less optical intensity than optical tweezers. Using an incoherent light source (a light emitting diode (LED) or a halogen lamp) and a digital micromirror spatial light modulator, we have demonstrated parallel manipulation of 31,000 particle traps on a 1.3 × 1.0 mm2 area. With direct optical imaging control, multiple manipulation functions are combined to achieve complex, multi-step manipulation protocols.

 

Biography

Pei-Yu Chiou received his Ph.D. degree in Electrical Engineering and Computer Sciences Department from University of California at Berkeley in 2005. He received his M.S. degree in Electrical Engineering Department from University of California at Los Angeles and B.S. degree in Mechanical Engineering Department from National Taiwan University in Taiwan. He joined the faculty at UCLA in Mechanical and Aerospace Engineering Department in 2006 after post-doctoral work at UC Berkeley. He is also a member of California Nanosystems Institute (CNSI). His research interest is in optoelectronic devices, biophotonics, and BioMEMS. His invention of Optoelectronic Tweezers (OET) is selected by R&D Magazine and Micro/Nano Newsletter as representative of the best 25 micro- and nanotechnologies of 2006.

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